	   SnapGene      ÝGACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTGAATTCgccaccatgagtggacctttcccagcagagcctacaaaaggtcccctcgccatgcaacctgctccaaaagtgaacctcaaaaggacatcttcactggtgggccccacacaaagcttcttcatgagggaatcaaaggctttgggggctgtccaaatcatgaatggcctcttccatattaccctggggggactgctgatgatccccacaggggtcttcgcacccatctgtttgagtgtatggtaccctctctggggaggcattatgtacattatttcaggatcactcctggcagctgcagcagaaaaaacctccaggaagagtttggtcaaagcaaaagtgataatgagctctctaagcctctttgctgccatttctggaataattctttcaatcatggacatacttaacatgacactttctcattttttaaaaatgagaagactggagcttattcaaacttccaagccgtatgttgatatctacgactgtgaaccatctaattcctcagagaaaaactccccatctacacagtactgtaacagcattcagtctgtgttcttgggcattctgtcggcgatgctgatctctgccttcttccagaaacttgtgacagctggtattgtggagaatgagtggaaaagaatgtgtaccagatccaaatctaatgtggttctgctgtcagctggagaaaaaaatgagcagacgattaaaatgaaagaagaaatcattgagctaagtggagtatcttcccaaccaaagaatgaagaggaaattgaaattattccagtgcaggaggaagaagaagaagaagcagaaataaattttccagcacctccccaagagcaggaatccttgccagtggaaaatgagatcgctcctCTCGAGGATTACAAGGATGACGACGATAAGGGATCCagcgcctggtcccaccctcagtttgagaagggcggaggctctggcggcggaagcggaggatctgcttggagccacccccagttcgaaaagtgaTGAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC  7»   !               #                     $      *                  %   "                  "               !                                                             '            +      #   +             "            "      "      
                  %   !                   %   B   %      %            
               $                                    !   %                           	      	                        !      $                                      #                                             
                                    +   %         %   (                           %                !                                     
               #   +            (      %                                  '         #                     #          #      $            )   '                            )      )        ! š%&OPQh“9:;@ETUv¤­®R	S	|$ç$¦Ä1³d ²  š1›½ÙoR”%V_w#
Å‰§2´±‰Yøc0 · °i<A¯Ø`	’	
*z%Åe
x³ Ø �û'ÉIŒF�¥T	¥	E}Ù%èÁuGù]¹¡8 	 d -´¾p•TE{°MboWÎ	œ¨Æ.´3Z# Ò˜³æõ\è`–ö	n
…
Í
ºP|®¿äj²çuµù’1A  ‡œ­&eZx$
Ûíó*¶Ï c•¨µ—pDŠ¥d±‹ ›2Ú¥ëS$Kä	µ	Øƒ�ŠL  ±eøì)=B~ÙÞ“	€
È
+¡M{Æ;áŠ > E¿‡ °Û…šÔ*DiÆóQ ¸ mŽ5áa	™	¹	<
…&qß$;¢f›Úy´ g ÀjÜ3
Áppçf‘íÊ¨$ ½ ‚ÕJVY“ÇÕ~Úh3€·ºe8H~—ý>]º‡vBX¢9 � Ï@sÊ˜¸ÊGnv	�
å
F»&\6à* • U6Q}(P‚Š¦9	U	‘
Ù
TŸ±3;VoÃÌZk~év›Âv‘xú®^“V Ù Lü;�¦	4
qw«"ÐWr&Ôßñ£jºÅ e ––±NcŠX‘ouš�©ñ1¹69}
[ 
 ?qK”	FüDç/IP4—ƒ3Á n .µ¿¡Ö|$Ï	µÐ9“îãyI y -´ñUQrápG
‡Ç¦ªß±_©Ô�Ìy¾‡% © ÓMf“´·º½ÀF]éa¡
é
’þ»Ü®¯k³›¸×MÆ¶Åú»k" YÅ–ðHbi™ŸÆö€—†
Î
K™Q+m…ÀAè<z“¥B¢È ¬ “7�ça
o
©D½}!­52J— Aà‚›÷	­÷Äå»v{ 8 Ñ h[Ø‡y–	î_^·Ð6–	Í Þ ˆës ²	¥¶V˜KT{eŒ ð �Y[g	�	
%
.
L
dêRXq1‹™š"    ^®Þ%þ'fŸÜáô+yÊÓ!™©ýEPX¦²p‚°²Ãç   r~§ú´þV¶	È	¶ÖòÙ<‹,kÖ ž ê ì%3ùLdéO�HIò< ÷ œ3“ÛdTP|©å	XA
lbMN; š Yd)s¦âð~	”˜Ž,„Æ04S‡…E! ²…£ù3yó>CÜß£	â	l
ƒ
Ë
,ÊNp~"<¸ž¡sÈ< fÞé€÷…î”	˜*£ƒÒÊ ] _ Ü�Á*Ús
u
�
É
h|�¹âþF?Ì:  s «­×ím	¢ìÒÇv ‹q�†ÛÍ p &Â 	•Ñä„ôH FŽÀë°h»›ê+Ejå¾Ð L �·ÞNƒl~±¤
ì
ÍOÜ9IB†Õ€ÀÇÛR<› * Ô Óì#Dwˆ «° a ç p·ÅË	õ˜ré÷ð4ò$'Œ´ÂK¾G C ¹ ü 7â=
{
à‹£Þ„ö{œÎÛz À n�ÁÅ„{Ø@’Èº	ø‚Ì%z< `#ÉM  0…Ã6|2	b	š	×	ì	=j†Ýü'·t‚Ýë•)
g™Éµ h èÒÇ'PqèCJ uªýT·Ý>,C	w
½
s�ø3q5’A·ù+[ ¦ k½ 
ÂE4gî ç€ Á,4ûÑ	Š
Ò
�)Ÿ;Ë© kŒ	KWZ`|”ÅÓÖÁfi�¸¤/9|ƒ˜ˆ™@‰ w Ï Ö_
—¨4»:?{�wC£: ¾ å 5ƒ‚ìÛ–fç@õþ^¶Y u Å -»ÈÑç			w¿äîò¢ì-Ijx»Í7Ànº' ÐAt*±¹¿ÂS­ÃãHkéš
ž
â
æ
ly²¼æ'ïÓ¼]`v+.9ÂÉáx { ‘ ÎãP¨
Á
ð
	¸aìÎÙ'v£Ð‘( > ¥ ™¶ËoÊ­
±
õ
ù
ýo¡áôò	²7éïn ! ËqÕw	G¬äÝ¼+ k Ç Vz�)X¼Ûý;Bœs¡I
UWpÍé[lw«Ã’±wgyúÎöûÔs» † Z7~tô‰4<Äˆœ[Ö�¯ý0# Y – ² Ê JR	 Wk‹	:	o	 ²É&u=[³ÊÔwÖaùS�½ÿ_W+ 3 R /ÕóQƒ§	H	V	’
Ú
¿Zî@êä’îK[lŒ/nzÈïÏ÷nŒ”Fa Nˆ ; i6	+
rx#be!F¾ôAXsáaŽ«ò�²»õÆ%  H Ú “ç™Éïrº¾ïý-	§	Ñð H'ÕàVcÚ®*þpÏ Q¶<üå/¤¯Ù5
{�¬9ùdÀÉ£!¤7Ü" #M]kÇðöýŽÌ½		­	Á	#,8BM�¤†?¾õkT ¶ ™ng$H‹S¬_	‘	"
~
{æ²ˆ\¸/7w± ê¬Ñ—¯²ådJY›ìò´)ÎŽ¾ãKoC¤° f ¿ë(Ç
p:Óæ~" � ” ¼ —Om’�
œ
Ø
ä
pvžª°2º
7–ÓÞð
\†5u ,>³Õ“•ànæ0Þ°^58x’í " « @ÄL•ï
GehŽ¶¹¼¿ÅEš 
è
‘J¬Ã·º;LÄº4z¡  Ý $ý
QU0O˜±J™Â
 rOUcµ	èN“H„ r Û¢Ý€èxªò„l	¢	á	—¸Ñ�rpÇË9…Ì ) K o �¶Òë"Cvª}%£
ë
”N¿Úä½ B û /À×?{¶z
<¶Ü”ÝïÈõ3	zÁJÈ S¥¼Ð	‰
¼
Ñ
 4:æ* ‹�,_ÄÐë 		v¾ –§•ñ9¡‚µz¿˜ˆ% z )°ÁâµRÂÔâOj™
¬
À
á
ô
«àëñ±ÍØ_u*-¢ãwîm! … .y�»òVüAs G	H
ˆ¾Èè²ÕZ`vÕ‹mÇõöü`Ÿ G "µûhq£½½óª¿bÙ­± Ž “ ¾é#GêR!
	o•ÒÝïB‡£·t¯.6 ª ÔNdg�”µ¸»¾ßb™å	’Ý¯ÂTG¹ìKN Úê¤Üçwƒˆ
¢
Ð
ê
“M·3œÇÙÆ¼À)„Ë  F „ (xŽáúgÁ^ÏF	«
ó
½ÿ•¼8ê°¾l´ôØ)û—l% ½ÛèàæcŒ‚˜‡
ª
Ï
ò
¼Lné¶½”ÁÑÜîSëA¢Ø–ƒ-ÊB } Ðã¼ÚçÆÇÈåEF—˜IJKL]÷�„	…	†	@
A
B
T
U
º»RÐÛìí%&éê=>?@{ ¡ÀÁ“”•‚”¦ *+,£¤É% ~ H	ÑäZ0G*M^jš ÇËø	M	C
V
S,åù†'»ÂÎ!C¥­ ÂˆÉ_™ñ–‡	ñ	š-B	|ç¨•§©% I{­ 
~ºå8îH0N_�ù
D
p
ªcT¾-Ü6ÍÃüx"D˜ x–_ÈgÞ	Eæî�¥6^h3Kb¦®  ¡ [”‹›¡ÌW
b
f
¸
 \ú‡˜ã¼îí . ­ Ò1+kèN	R~j"®(ÏÓ
 a�Ö\
*¹w!m– ‹ í.²ø	û	Q
K›¢Ål¼ª >Ãð:‰`šòˆ	ò	2®øÇ.Ù-¨}èNÒ|¨ã BÐáÊƒˆ—œ
Ã
CæC?
©%Ò  +J®/c»9…"ˆ—	RX¸1KU¿ÝnTØÞ$ § |'Ÿ5\ï1IO`‘úˆE
ïÿ?«Ñ.k—Î•Äáy€… Ò ƒæžn1q
7CÈ‚ŠÎE™ 9 åiÌÙqz¦
¯
î
÷
j`_d7¯ýü
># @  ‹—‰lh³	ß	“ïtÅ17u¯ <y&ìÉ!
Úç´·QL¦ëf4§ ß ')ã$EtÏF¦Ýßc‘ á `(qK¥žW™U|_iLR� ¢ í ô o¤÷±ûoœ\9
¨ÐˆäDï-�h} ñ ÿ P]\G›¤Z¢ÌŽ	c
¹
àr2š½ëe € ¹ž§
>Íh	
&
/
M
X
]fÕSûÂŒ$›p“�À •[Œ¸Í¥/4
g
FŒ7ŠeëY™\î.  WVÿO	ª	õSÇž£iÞö #)1S9±! 	×à{Ó2¯l âUËãH‹Kô3@kˆ¯ �ÐŸqÑŒt%  / N ® Hïwv‡¢ß,€gÁ
³
û
Ý,6Ô"ª;×ÉFY§Ôf³èñ  _&é(³'ÑQzšŽ¦&þQ³-ØâƒÄ  Ö C½˜×t	·	×Ù ¬bWa? bbzW·ÔÚN§LxRn s¨ûLµ0	ž	É	Õ	ó®‚"—× ã ‘îÿ¹å]
s=Œ+º-Ì5lŠD e¨³úMêLªIm�½ó3 , ‰ Œ Ÿ îºeù	R
IPã÷ – #« ë :Õ%Fyí¢&/œ‘£ÞÆ~s�¾	 4¦÷ü	žJ²= ÄGNõUQ}­ªøó	B¯ûùmò_Yfæöä	 ø ;iYN.©´O ?�Ë4<ÜŠ*6aóù‰	ÈOéÓ}ø # ”ñe²›òæ	þ	3²/Úc�~<Ô!©×NT › Zñ•…Ç1^Mç”~�Fk¸ `Íœ„!‰�Ä
�’5Yùcª&þ *§ÑâË×y	™;‚DT	›á­Ò e¬CtJsŠ9u˜ã	
-ŸÂDˆ@†Óß ¯0€¼ú$N’È8?D^u€£¤	)DyÀ#tÃFø	! c ,³†¤#SDzônçÍ	ã	õ	m
„
Ì
§-¹×Ob”³it‘  d÷†Ûÿ42Ýà˜	;
LÞ#ohŸÅò�ìÉ=PÙ$ KTÎ?rÉ5P|:·U‰SËÏY2Vqš¶dü=¹[¢iUÄß  m  ~ðJPqûC‰#�F
†™ðÏÓË8–â	2À† ¨ ’ß6�a’ž¨¬ý­l„ šÖÅIj– 7 ï gZ]êrÝ†žŒ	•	±	
K
à ^]xÒ¤˜üWz�æ  ™ é ö Xq}¦ù(’³ý2øáïÇ	W@+Å
/ajñ:Õ \ ^ Àö2¬Ûk
r
t
‚
Ê
ëo}!€‚ÉýÿŽ Ó Ý‚Ÿ¯ÁÌ8HºÐãƒG;Ïš ` „ÄzoÂÄÇë	iûËyŠ�"ñ&‹Í½F ç+Ñ=ú+v
rŽœ÷2Æ(Dž@¶ø t Ä j{ãeæë?i{wÌ?   ¤ Í¾¬§
°
ï
ø
k·±`nÒ&uÏ�èÜ 2 Q X ± É IÔWÚ:jrn	Yí?<ªÓ°ùŒÓþr/M‡# : Ææäî®¹ËØîü*
€a£ E…8@GÈôŒ6ô)ýS$Î µ » ˜m'«^	�	}
¯	Òå…° =´	]¶°Iÿ  A q ú ŒRµk	¡	à	»
–28 !Š€iÃ	”ðYuÆtõžv E ƒ '�ùv"Î 
F†¿5 GÁ‡D\Ê•L	?
èµ¼ëRº¿Í×æ§�¨ z^íŠ/Ý	e
[ÛiÌâœíìg =�ì9-±P
[
1Ø¸,k§MÑ  *‚ä.„�m‡¥
í
i>j�àSÝ= à (#%k
ÎÙJPÜÞ ó þ ¸öO\F¦Z¤3
8
e‹6§ÏCÁ#êdg|’œ M ßUu²
ÐÆPGŠÝÿ:?�‡¥Ö%0RÈœž×Ðs Õ â B¼íKayäs	rØ¦Ÿ«4aQV` + ˆ 9Ô$Ex¹¡¥öâ�±2}Ž
 ;M)¬±õ ÷MS _I‰ �žLX“šàøb}j· b Í>qÈ#ÚÇm]Ì	:
¦(™µscEÄñ�ëOÃ� ˜ è î õ p¥ø‘²ü}qp�Bà‹	Æ	
w	7™ûV¿Hi• [ ÃõÆê	Ë�öÅÊ '‰!ð%�µ~ £ Ã í¸Êû)
¶â>?©Ñåê>ø%5ó(.LR ´ º Q]	j	�	|
º
s�Ÿ D ‚  ]†Œ,C[”ÍÜ	>
O
Z
d
á…¹¾Ìì€ ò ý -Tœ¥Y£
dhÎØ>Œ¤$Q›�ÖÏÜfr{ 8A»^LHãr	×á’ß3÷a|ô¶ö Â p�ÂÇ"|ÙAÉÅ	
(
¥µŒ&=Ã $UŽN” � º…B“\	i	q	Û	
N
Y
cgÖÍ¸ËœÕõqž+ Á oÆ?@AÎ»	¼	
'
^_`ab¢£¤´ÖŠ‹]ƒ\�·Êô\]% Ÿ6ŸÞª¨8Ïÿ		'	<	½	0
×ù^T„ü{ûa¦¿]%ú^Á Ì –n}	3	¾	1
Øú8‹ýì(Uy»u®Þìä&¥�Ê ò©\¹›	fýª*Y]ëû_% 9ÄÎè7²05�	(	Ø	
h
G‡_üg¸§À–^ï/š( 1† 7 ß«Ac�ëƒ¦ÐÜ 	#	=	c	í	>�kÞÌZ…|šüOUbƒhX¶Â  ÓP	
2
Ù9T´z$nv¯ßå¦ž W	~§|	ðŒ¤Èj)VQ*Ë éöÈ%(Ð€rté2'D:HK²Ù i Í X—Ço 	+	4	«	¿	ûŸìþßí÷¼YeíT ' ª‘º?=¡x
¾
tÌäŒ‰°6Úp ^¸ìu 
v«Øþ|¸ÔåÞ]D	 
¶
þ
ã�4’1IžÑr#\­` áU3 <m-4VIg­1AlrÃ“+ZÖú,Š�Pƒ –gó°œ	ù9Lõþ”Üð]Ž!Ê€«ŽBÒü% 0 5 O ¯ 3x�¾Ïâ8ShÂ!6Ži
~„Þ\Õ`!FÊÑhïg0 I!÷wˆ£-³1…©J	
ýhW_Õ!è  ð?lÿÅé�	Ù	è	
ˆÃ¹<M5—ùZ¨òP›´éòV  % §1Žõ:à)		
´
ü
H-7#«|¨ÁØGð«� 2ód	 5?Jlž{†�P„ 'ÿÙc­·! ¡¡©à×-BÎdì'§Ñ)Ò	î	ŽÒR[ž*›ý§ñ<.–ßãÅ 8´ŽêÝü	$	ß/BÍ›}V~R´Ì¢ªY    T ‡¬Â`�„Ú5Ñô´	>	X	‹
”
Ó
Ü
(Áæcpª>i„Ã  × ™Ø×Q	ÄØ#ˆ¥0°cX÷bŸ  L[a•Úµ‚{­–æ´@Š D¾l�
}¸	
„ ÀÚ:o„™¡à)C‰eš§ ÔXÆÔ8	u	3
!:UnÂgj}u¹�%w}­’AW x 
t¸H¥©O¨€|‚½xH X`
˜ÛöC¼*@y¤Ç  Ð ×Xf	-
É5éWSo¯Ì � cc{¨}	—Õñ�k3;R+G†MD; „ÖígÉ)¢Ñu·E;Ú
 €MêkŸ	¯gAé ¿ æ 6ƒ‘1	Ê	Ö	÷Üô—�èsöÿ_êƒ(#L³˜ t©ü¶B	&O3BIZØ v Î ä 4ºÒæ^
Àí£,.ô6€m¹ =  pÉèüxå îó»8ÁÈ'»# j Æ Y›ó		%tZ‡Éã‘íøJRZfk.y�¼ÍîÎm‹Eº+ P\j’ïõ˜È.¼	
	,	5	¬	À	"+7ALzd€ïÿàø>`c�½¢ UÛo f«Ð®äIl�
›
Æ
×
ã
ëz³(Íå�±½âJ[n4v Ü +²Ã’î#TÄŸ
ç
m�M«7w‘/:˜Ã¹y ( J Ñ!Bu©»©ñ>zy
;ÿÛ¾ÜqôoåÊÇ( ¯ºÀ´û@Ó+¢¼NŸêÿ˜
¿
à
u½çòð ÔŠ×^a,¡¬°_âí  � ’ Ùé£wfÞQE	ä‘”72ó(Js(®% | Ïâ»Ùæ/ßä^)‹ƒ	ð	S
©
ñ
¹äø,“ÏÚ$Ÿ’)¬ -   w•¬ÿ}¹f·
ÿ
Qbí5—Òûw¤]a Š `ÕïÏ‡ú	
Â

JBÆ¡)>Ñ l$â ¦ &bžË4!0p®
ö
QþW0J®BmÇ”‰×û„ ? &;ŠâD'p’s¤³Ä0êtQ� ì n£°úš£	=·Ìn.ËEÔ‰
ß,[-Žo¿  ÖGVzîv†¡À©	²
ú
&5T�¢âhõJó2,eá8 ‹ð —¸/	�	Ô	Ó­MËK�‰C> d§Höÿ•�ŸÝ©"r½ " ÊFhô±5ñ÷øý	úñ^�Xe³ÓåÖ «Ì›rÖ8tx	:‘]Á¬�ÞýÑ' S4O{öM‘¶RþC1Tæ7t¢ô	CÎÖax"“²Âûh÷Zgh 6 l W‘YÜ�"�°	J
V…ß]§ü\¬Ä`Õið91åÔ *y~®¿Ð<«ÃùÀj
q›ê1únGx€�Ïü÷¼# 1 P W ° È ÅHÓV½ã9iX°Dm„;Fóhz‹ËÒtÎç ù <´ª	®X\µ¯þ 8ø‰.uK	0ZË×¦Ö  õ["j2†

7
Š5=Å‰þiO/"é ‡ :J¸x¤õ€�Žª°`P1 Z — Ì=ÆôìlßúŠ	é	Ê'á=vÄ>´Éb6˜úêQ ³ @,KS‹+X×=‘#P `óµ# ž5Ý©! 7þ		&	;	[	p	Ä	Ú	
¡³‰\Õú¥¾T[¶Ô~óù[  Ë ñ
@mbçê‚±ÛŒ	"	ËûfxºNT­ã©X¤œêW Æ¦*	{	

ï³ë$ÏXdmsGØ# & •fä;�<ÿ	µ
ý
8¬0“Ûï\�‘0ÂÉÙHo�Õ"‰¬O‚ $ 4 02>��ôöþáR„¨I	ç	}ƒ[ VL{ÐøñOªU$  S ¨òÖ³ÍœÙÐó	W	“
Û
4IÀ.Aå�o}©ð=•Þb¡¬ 7	Ã mƒŸ"|¿t‡�¬•(B ‘³ödV  œ e	,
s–GõBè2F…¼®GÆ j�ÕA	f¡NèA) < 3O[iôšò	!*6@Ky$cY†Èâ_b�÷QóŽ¡ÚÇl¹&¸' I Û  ®Ïí"¨Òð*y»žþŽ
—
Å
Ö
ß
:�ñŸþÖáZÛn—«¯äÆÿ _”è¢ÎávØ.e(†ŠÝ‚	ï	“6AÅ+(ò=úk'á  %m‰¢a¯ù�ÊCu… ¿¨	%DPÓSˆ/I/dà+ˆÿ ÉªEš c7sðö·.	Ó	Ò�\Àœ¨WdˆqÕÐ V RÒLµ0å¿¯	š¦0Cƒ:E~Á
gŠœ8ÍÓ  ô9·	
6
/­Ê¥® +¨æë°ÚÞý	!	%	Z	Ã	à<úe¤¬"âSµ£Z Åã=ŒÌý�Ø;¥Ïz	|‚ÎœUWrÝ«  Nh�™Ô	@	)Â‚ çöEA@UÅ) $^lˆÈ®øDu­ÿbtŸ…Ü6xõ¶�	�
–
Õ
Þ
$9CO‡.@¿Äm‡j‡Ôà  U óÄK‹:Îä¾	 	Y	®	Â	
.¥fq‰«)  fgñò­÷ÃþažÛvwÍÒÓµ?	€	Œ
•
Ô
Ý
-NÃde	
?l…†Ä  ,¬  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                        )                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            	                                                                                                                                                                                                                                      !                                                                                                     
                                                                                                                *      
      	                                 
                        
                        	                        )         !         "            s   Q                     O                                                                                                                                                                   )                                                                                                                                                                                                                                                                                                          	                                                                                    !                                   
                                        *  
  	           
        
        	        )   !   "    s Q       O   nJ  od  o|  o¨  s–  wÆ  }fÈ	”,**�	$·a‹ŒÝ*¾
Kf†Ÿ(ÖèŽ¥;ž
æ
Î	J +a+òŸa
“DÓMÇ²Ò”uØ%î%ØîÎ	3¨
ð
·¾ MÇçkƒ� ¹óQWs‹ÈÊô	-	¨
ð
#rxpÄÛflá4?Àì@ƒõ.²}é¼3þ +;…á $+I; +;þ+  +Üb08×>0Ž
Ö
ávÏ	Áq.Ï(00 áÊÓ ªù„’Ø² KÜ®‚Ä
¿û‹ÍÜÙÞ­M¦çKÎ†Î¿
Øªr§ú´ÖIPÙãÑ‚D‡ªÊ‡ìv	~¡Æ	â��‡!–!ÏVq%Ä~ “ËV
ùü¬Õêr§ú´~<‹XÖ‹ã Æø  -ñìª †Û  ÛÂûŒÿ \c
àë­}(~ ûŒE¢ ÛÏ&-ûñŒ�¥T	èMÏsØ~Mako­
õ
±O—‹žàBx9mj½À°ÏŽ1, 		~Hº ÊÀûü‡&3oŒìU	a	v	‘
Ù
~¡Æäj“f´RC²Í�•60H¬¯qƒK�	­aKLä�
Õ
Ps­Â"/Áf¶Ëž”'cav,6	¾sŽ�š!­–
Þ
s¡wö«!o¯DaP/Í  -ûñŒ�¥T	E¢ìªè †ÛŸ3“ƒÆIaP­/>LUÓ“Ü®w Mz~ºkoý	6	š
­
â
õ
Í¨êð9=sŽõÕú'®aP/o­
õ
ÆK $&eÛ* ¨DO¥±±ÂÏ Ö4V©	�õÉFñåÂ…B“¸ËJY“€ó 5?J Ù-ñsRCü¬²ÕÍ�•ê¯À˜
à
^,V9Ò“¯
÷
âç PÙ ç À£Þ€š	Ñ	â	IP åÛÙ  Í 3ªìŽã�ÌÕ(`ŒæL¯5—ùI“–ƒ  ã „<a Ë	6	sŽºû@šŸç¹rF»½°ºû@ŸçPÙ‹06H¬bÈš ”0ErÞ
n¦¯)y	) Ò"CvŸª5|à;
šÞÙ ­&eÛó* ¨D¥± ^%'y™ýP²‚ÃRLµÏ0åŽ
Å
Ö
ÁáZg¦€qçƒÇ ¹¼¿éW¼ælïÓgy]`+sáØ L¥Àûü3Œ�¥	ƒj¹Ê¨ÂÃ‚"‡AöUe“	9«Žü¥À3ƒÊ¨¹¿é¼æïÓ]`+áÇ ¼Wlgysu… ¿¨	%SIdàÿ–	·t	¬ie; Ç ¹¼¿WÅÓéx#Wb¼ÁæfilX¸áïaÓgy|ò�]`+sÆá@%RLµÏ0åŽ
Å
Ö
ÂÃ‚"‡ÁáZgAöU±
ù
¡áôò7ï8 hØy_^6	NõQó	BYfI  ¨ðy:þÛnäÆxí¶Ï•&,á & �<Ùol "�…1š1ÙR‰Á4Š
Ò
C½}¨­ªø·	ùmÙM ç½óö& l ³ ø�u<"�…Ùo1©ñqoÊFô÷úñ^Xeåþ<‹,kYNh™	)öÖ¤/¬ “dçÏ	å	dêX}!b­9;Õñ&;O+BIØÛ5 ‘!Žd	„ÙÞ\´z6„åhï­¸0æ°Ú!	úe¬â£Fé¶7Žë°÷âîø*à+jÞöA{Ð© ÓMf“´·º½aÜ®¸Mð?lé�	¹M¨›éVÏ	9“då	b;÷îø*A¬ çdêX}!­; WÅÓx#bÁfiX¸áa|ò�Æ@%Fô©ñ÷úñ^XeqoÊå§ 5`‘«kÄf*Y ?v Î ä 4ºÒæ^
Àí£,.ô6€m¹< 3O[iôšò	!*6@Ky$cY†Èâ_b�÷QóŽ¡ÚÇl¹&¸kŒ	KWZ`|”ÅÓÖÁfi�¸¤/9|ƒ˜ˆ™@‰* Ô Óì#Dwˆ «°… .y�»òVüAs G	H
ˆ¾Èè²ÕZ`vÕ‹mÇõöü`Ÿu Å -»ÈÑç			w¿äîò¢ì-Ijx»Í7Ànºù <´ª	®X\µ¯þ   ^®Þ%þ'fŸÜáô+yÊÓ!™©ýEPX¦²p‚°²ÃçÙ Lü;�¦	4
qw«"ÐWr&Ôßñ£jºÅ7	Ã mƒŸ"|¿t‡�¬•(B ‘³ödV¦ &bžË4!0p®
ö
QþW0J®BmÇ”‰×û„G … • Ù "LU.6Qy}�µü(;»òûVhüAsPq�£½‚Š ¦9	G	U	¦	4
H
‘
Ù
TqwˆŸ«±¾È"3;VoÃÌÐèZk~½éóWrv›²Âv‘ÕZ`vª¿Õ&x‹ÔßñmÇõúb£®Ùö^j“­±ºüV`ŸÅ ? ¦ à &(*;‚Šâä&.b#%kžË4D„�!'0mp‡
¥
®
í
ö
QiÎþ>Wp0J’j®ÙBJms¤³ÇPÄ�”ÜÞà0S‰×Ýêû=tQ„�s¨ûLµ0	ž	É	Õ	ó®‚"—×  ¤ Í¾¬§
°
ï
ø
k·±`nÒ&uÏ�èÜ ¡ [”‹›¡ÌW
b
f
¸
 \ú‡˜ã¼îíÅã=ŒÌý�Ø;¥Ïz	|‚ÎœUWrÝ«e ––±NcŠX‘ouš�©ñ1¹69}
[D ‚  ]†Œ,C[”ÍÜ	>
O
Z
d
á…¹¾Ìì€f … s¨û*Lµ.y�§¿Ñâ»ËòVüAs×ë( 0	G	y	ž	É	Õ	H
Ç
ˆ¾È™èó;‚®²DpÕTZ`vÕ	‹:m›ÇÓæõ~áö‚­ü"`Ÿ—Ò×W	~§|	ðŒ¤Èj)VQ*ËÚ  -¿      t  E        E¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥8ZÏ}Œ;øˆ¸nŽAó¦OÔDx¾ÝU)îøöúÜaß�=ÑStôþqô÷C÷ß}-ÁX·JÝôï÷^-í—ø÷ß}-€X÷Z»cÝ*ïßu`Þ9ä½FûÞ(?·ø~÷û>KÔ/PºyÜ€õÃ¿O¢+Nõ?VµoùŸÖ´T: Ù¸ªýu_P½
!Âo÷âîí«é�Ó§êµt�´OÄ�.~�ƒuˆ¾¿t4Ý v¶Ñknë;ÔïèTëŽ V56JêïN+~‡ã»¹C�;N>@w¬b®è×È}/îŽ>í¶ƒà¾û¾ßàŽÛ>¸Û4qÖ×çÓþûiÔqÆPÓ>÷ûJ„P0C¸0ÁLn:÷ÿJÅR@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ør  ™  p  •  ñ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Cd68-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2034" maxFusedRunOn="2034"><Segment range="928-1905" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2034" maxFusedRunOn="2034" detectionMode="exactProteinMatch"><Segment range="1912-1935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="Twin-Strep-tag" directionality="1" translationMW="3045.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2034" maxFusedRunOn="2034" detectionMode="exactProteinMatch"><Segment range="1942-1947" color="#cc99b2" type="standard" translated="1"/><Segment name="Strep-Tag II" range="1948-1971" color="#cc99b2" type="standard" translated="1"/><Segment range="1972-2007" color="#cc99b2" type="standard" translated="1"/><Segment name="Strep-Tag II" range="2008-2031" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;two Strep-TagÂ®II moieties connected by a linker for enhanced binding to Strep-TactinÂ®,&amp;nbsp;an engineered form of streptavidin (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/24012791&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/24012791&quot;&gt;Schmidt et al., 2013&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4339-4355" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4408-4429" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4363-4369" color="#ffffff" type="standard"/><Segment range="4370-4387" color="#ffffff" type="standard"/><Segment name="-35" range="4388-4393" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6334-6438" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4145-4266" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2961-3096" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2069-2293" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2781-3110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2339-2767" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-5302" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3177-3971" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6264" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5473-6264" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6265-6333" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>21d8eba0-d307-4bd3-939b-e32c871fc55d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2024.8.15</Created>
<LastModified UTC="9:5:17">2025.11.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..924" colors="black"/><ColorRange range="925..932" colors="green"/><ColorRange range="933..1904" colors="black"/><ColorRange range="1905..1910" colors="blue"/><ColorRange range="1911..6468" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..924" colors="black"/><ColorRange range="925..932" colors="green"/><ColorRange range="933..1904" colors="black"/><ColorRange range="1905..1910" colors="blue"/><ColorRange range="1911..6468" colors="black"/></BottomStrand></StrandColors>  €ý7zXZ  æÖ´F !   t/å£à/>] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x�ösôœ·?ˆtÑí«úÅD›$P8+³"N
ì×ö•Y*¡Ç÷{ù.·I/1núä1o=±	ÅS¡ð2ÞÙæ‚_©<pš1X«ÛærÀP qâõ‰BÎGF™±!ª²ì—“n^°E®oŸh»ihU?²:}˜†fA£gq#üìhCçi/áâ¥ê†—•ÐÿBÿKZý@µ°ó„fŽ3Ì;2ôF?è¡¼©ª»OžÏà±Ú�i+'Øá7”�-£‡Õ:¼lÌ!² ùýF ÎªP”±ÄÓC“ñ7£ôo'±Øk )FD�‹9f›ûc¡æ)“¨º7Y(Äk ù'?‹=ºL›VÄË½N%ßb¯ü%]¼5Ü…^“zÝ˜ÅŸ¹“ÞER!/.é�ÑÖõŸÊqâL�©ÑñY˜9ÒL:Hì(¼þÉåV}b›¢]È@òï·�î…¾ûé/Êñ¦u­IÙ´f9+�Çç‚÷R~FfýCT€"ÒÏ.åõ$¢a@¤ 0G_¼î°|Þ)i-—Ú‡l– k®EZ¶Ö{êw4êáÒ·)y™R²q
çýÅ.ÛsŽ°íö{•Š½\¿QæS|­íwÑ8i‹sÚ_]²ZtO?DAõ=Ïüý�Ï«[ùÚ%«ˆ`LÌù{æñçQ•"ëv/5ÁAô`k°¯d¥ti0Ù¹¦güÃ[{ŒÛÚ-ªÇ'CÏÁ>MEKá•>÷7d0ŠSlÏý)ÒÕÇ'j<» ÁnœICŒÑí¾Ñ^Vøèi£†/ð»åê¯Ê¢@”ˆ–»Ó2ËônÉLÕÊ¹ù�ì›#NÄI_3±ô^oç�Â5¤âìßEÝ>àJðœçªêôÖw
¡¦7`²2ÃsÍÃìê½ñ¯#qnÞ-èÊ–</±Jò£ÜSõ ì™1ã©i!*ßi†"¢/BÍÞQŸ/øPéše'DîÐÃ!º÷Ò>(cŠîÝÉ÷DdÛXý–çÂî¥ÿmÖ!Û A/õ�FÓ�Oe2ûo|ÀšLŠ5rþ•á‰1?¬ý:	�!ð\
1SØ¸cXx�-PqVYî~/•¤ÒñÁ°Êj»?f[T~Dö¿–Ž§››Ö÷\Âv*_¼û‚t,ø#qÕ70ßÛ„£±ÔKqö'g�ÊAlFáy†o%Ž‘¢Û%zÄÛûÒ²ÈƒÛL¥?2¿™³¢B‘(jÑë,a0ZímoÙM×“^k/·L¢GæËî¢³*ò„8ã~×~‹§°)ß`¼‚UCP(Fó4ù„�¿‘åq7«åµøalî|§5 î¤*@Ãï?½�Sfs^7tUê.É©VsòÕª
èÅ­Y·§hë¸	ûŸ¹È‰×¤Åmši„Ý•?{(Q2g	ìüïZ¨æŠN…Gfnª˜:Ãßn@=²�k~Díâ?øƒçÎ	I³‘­a‘ûU°Wûãà @×µ£­ˆYÛZZµÿ ¤„ï¢WÄ”²áPj5ÂDµ¶ˆýp1mu*v}‰*T4Ä¶©Pæ…�úO^ âž¿•åýB?Í¡óÒ¨ž©Áa€±„S?jreFfhJƒ'KmÙ/ÖA' ³ƒ}_Sj7²íïH¨�U_•¯áGeÍ6à8—­¯©úF¸‹íg2#Ìõå‚43m0Î
óx›ËÝØÅŽªŽgÏÏj@hSVZç›­ÇËgš,ú¤ÿ—¦:øåÙ~|ì!ËÃ~¼˜hÁd„¢¦ÿç§³Ú€Ù@„lþ0W³¸é_*ÔÝÂ•µ�>š¯�B•d×ÙWk4&ÚTa&I-%€£ŽÝBŽ¿â|œ ×b$Ò[Ä!zb`†´]¿¿½Ð£«½°àõŠ�¤‘è�Çù‚Ÿ"í<§¦UÛVd®Kê.Þ¯ò®z0Ø¡ÒÔÂŒôð-ÓøÃ_XþKÍÚ`ýõì×w¥›Ü‹j€�L ¾›'Sx�rê÷1ÿÞ�ªàëÑÐ±—£7™–Û™A îN-ét�TcÒ{Š/Ê_K)21Ë­ÉêE`¯	F©�È»z(ÅÜalCˆ/P¯FŽöÿ­Sík!’¿U*Û-Ä]MiA“Ö¢Iý+¼ÙÄM.¨…^«˜Ì—4aAF;{Þ§í¢ÞfkÖÚîôL{¼]õ'¯öÒ{ÏSéÖ<šI*ƒ—�Ê¬kyr¡º•e>Æ}�4[L„,ºP¦^œ(eo+öä¤%ø=´ÿ>�i1 \…Ò…$tS–P?-°T"²Øšùû?kHÑÐ%)gHvéqËÀ¤šj:…¨.ÍðŠ�™(üµR–^w„6‹¤`ÿ�‘Pà<‘�sœî¡3ü¬ô„Ä”DwïÌ }ý t/ì•‘è3äÆ ™uÄ3Ý™Éä)[ŸÁúrÝ­éŠÞèueÓ£yk¦{õ]yZxaÁ24xŠw™:èL9•VÔ$êlòn­Ûº˜þÖ„Áxò¾;72äŸ-ÂÐë–~Ÿ€�™êÝÄYDA,›ŠŒ Ó¨‘À+ìÄÚüGEb¯6HMØfU:�%™¹ Í52!=jì"§­SV¼¨p˜� ²}øåû~Ù“†àñË›5ÜD›Í­…çç*¾³î	úCÏ}¦W­=Ç‘*dUÀ1NƒL¤�0æIÚ�ÆÏGA¬€YÊnè¦éiôqh(‰…Pý0WP¥     è9ÞORuÄ Ú°<  x. ±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &O<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="Ms4a1-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1929" maxFusedRunOn="1929"><Segment range="928-1800" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSGPFPAEPTKGPLAMQPAPKVNLKRTSSLVGPTQSFFMRESKALGAVQIMNGLFHITLGGLLMIPTGVFAPICLSVWYPLWGGIMYIISGSLLAAAAEKTSRKSLVKAKVIMSSLSLFAAISGIILSIMDILNMTLSHFLKMRRLELIQTSKPYVDIYDCEPSNSSEKNSPSTQYCNSIQSVFLGILSAMLISAFFQKLVTAGIVENEWKRMCTRSKSNVVLLSAGEKNEQTIKMKEEIIELSGVSSQPKNEEEIEIIPVQEEEEEEAEINFPAPPQEQESLPVENEIAP"/></Q></Feature><Feature recentID="1" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1929" maxFusedRunOn="1929" detectionMode="exactProteinMatch"><Segment range="1807-1830" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature recentID="2" name="Twin-Strep-tag" directionality="1" translationMW="3045.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1929" maxFusedRunOn="1929" detectionMode="exactProteinMatch"><Segment range="1837-1842" color="#cc99b2" type="standard" translated="1"/><Segment name="Strep-Tag II" range="1843-1866" color="#cc99b2" type="standard" translated="1"/><Segment range="1867-1902" color="#cc99b2" type="standard" translated="1"/><Segment name="Strep-Tag II" range="1903-1926" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;two Strep-TagÂ®II moieties connected by a linker for enhanced binding to Strep-TactinÂ®,&amp;nbsp;an engineered form of streptavidin (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/24012791&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/24012791&quot;&gt;Schmidt et al., 2013&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="SA,WSHPQFEK,GGGSGGGSGGSA,WSHPQFEK"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4234-4250" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4303-4324" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4258-4264" color="#ffffff" type="standard"/><Segment range="4265-4282" color="#ffffff" type="standard"/><Segment name="-35" range="4283-4288" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6229-6333" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4040-4161" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2856-2991" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1964-2188" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2676-3005" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2662" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4612-5197" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3072-3866" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6159" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5368-6159" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6160-6228" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¯<Notes>
<UUID>21d8eba0-d307-4bd3-939b-e32c871fc55d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-Ms4a1(mouse)-FLAG-TwinStrep-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2024.8.15</Created>
<LastModified UTC="14:0:24">2026.3.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             a<StrandColors><TopStrand><ColorRange range="0..952" colors="black"/><ColorRange range="1800..1805" colors="blue"/><ColorRange range="1806..6363" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..952" colors="black"/><ColorRange range="1800..1805" colors="blue"/><ColorRange range="1806..6363" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
